Figure 3.

Clonal geographic distribution in bone marrow

(A) Heatmaps depicting the contributions of the top 10 clones in purified left (L) and right (R) BM CD34⁺ cells and concurrently sampled PB Gr over time (m: months post-transplantation) for the three busulfan-conditioned animals and one representative TBI-conditioned animal (ZJ31). The heatmaps were generated as detailed in Figure 2A. (B) Pairwise Pearson correlation coefficients comparing clonal contributions retrieved from left (L) and right (R) BM CD34⁺ cells and concurrent PB Gr over time (m: months post-transplantation) for each animal. The color scale for r values is shown on the right. (C) Fold bias line graphs showing the fractional bias between left and right BM CD34⁺ HSPC clones over time for the three busulfan animals and one representative TBI animal (ZJ31) over time. Each time point is shown by a different color line as indicated on the right (in months). The x axis shows the fractional bias comparing left and right BM CD34⁺ clonal contributions. The y axis shows the cumulative percentage contributions of these clones to overall retrieved barcodes within the two samples, with the maximal possible contribution of 200%; −2 to 2 regions on the x axis indicate nonbiased clones.