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. 2022 Dec 15;12:1042779. doi: 10.3389/fcimb.2022.1042779

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Copyright © 2022 Wang, Chen, Wang, Guo, Peng, Liu, Li and Du

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphology and H&E of palate shelf tissues at E16.5. (A, C) The palatal shelf contacted the midline and fused through the formation of the midline epithelial seam (MES) in the mid-anterior region of a control embryo. (B, D) Unfused, separated palatal shelf from an embryo of RA-treated mouse. (A, B) Morphological specimens (magnification ×100, scale bar 100 μm); (C, D), H&E staining results (magnification ×40, scale bar 200 μm). PS, palatal shelf; T, tongue; NS, nasal septum; H&E, hematoxylin, and eosin.