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. 2022 Dec 15;9:1076073. doi: 10.3389/fnut.2022.1076073

TABLE 3.

Primer sequences of target proteins used in real-time PCR mRNA analysis.

Protein name Abbreviation Oligo name Size Sequence (5′ to 3′)
Actin Actin mACTINm422-F
mACTINm496-R
74 AACCGTGAAAAGATGACCCAGAT
CACAGCCTGGATGGCTACGT
Fatty acid synthase Fasn mFasn-m7703-F
mFasn-m7904-R
201 CTCCGTGGACCTTATCACTA
CTGGGAGAGGTTGTAGTCAG
Glycogen synthase Gys3 mGys3-m681-F
mGys3-m740-R
60 TCCTCACCACCTGGTTCCT
CCACATACGGCTTCTCTTCG
Glucose-6-phosphatase G6pc mG6pc-m153-F
mG6pc-m737-R
484 GGTTCATCCTTGTGTCTGTGATTG
AATGCCTGACAAGACTCCAGCC
Phosphoenolpyruvate carboxykinase Pck1 mpepk-m195-F
mpepk-m770-R
575 AGCCTCGACAGCCTGCCCCAGG
CCAGTTGTTGACCAAAGGCTTTT