Table 1.
Primers used for cloning and qRT−PCR of LV-ACE, LV-APN, LV-AT1R, and LV-RR.
| Primer name | Sequences (5′-3′) | Description | Tm (°C) | Product size |
|---|---|---|---|---|
| LV-ACE | Forward: CGCCTCCTGGAACTACGATTCC | For cloning | 59.5 | 2,326 Bp |
| Reverse: TGAACCACGAAGCTGACGAAGT | For cloning | 59.2 | ||
| LV-APN | Forward: TTGTTCTGCTCGCCCTCGGT | For cloning | 55.9 | 3,100 Bp |
| Reverse: GCCACTGCTCCTGTTCATAGCC | For cloning | 60.3 | ||
| LV-AT1R | Forward: TGTGGATGCCTCACCGACAGTT | For cloning | 60.8 | 593 Bp |
| Reverse: GCCGCACTAGCAGGTTGATGAC | For cloning | 62.0 | ||
| LV-RR | Forward: GCGTGCAACCCACCTTGATGAT | For cloning | 60.7 | 1,116 Bp |
| Reverse: ACGTCCAGGATCCATGGTAGCC | For cloning | 60.5 | ||
| LV-ACE-qRT | Forward: CGTGAGTCTGGAGGAGGAA | For qRT-PCR | 55.3 | |
| Reverse: GGTGATGTCGGAATCGTAGTT | For qRT-PCR | 54.8 | ||
| LV-APN-qRT | Forward: TCGTGAAGGTGACAAGGAG | For qRT-PCR | 54.0 | |
| Reverse: CGTGGTGCTGGAAGAGTT | For qRT-PCR | 54.7 | ||
| LV-AT1R-qRT | Forward: TGCACCACCGTGAGACCGAA | For qRT-PCR | 61.2 | |
| Reverse: GCCGCACTAGCAGGTTGATGAC | For qRT-PCR | 60.8 | ||
| LV-RR-qRT | Forward: GGTCCACCCTTTCATCCGAGGT | For qRT-PCR | 60.8 | |
| Reverse: GCAGCATCCACAAGAGCATCCA | For qRT-PCR | 60.2 | ||
| β-actin | Forward: CCACGAGACCACCTACAAC | For qRT-PCR | 54.8 | |
| Reverse: AGCGAGGGCAGTGATTTC | For qRT-PCR | 54.8 |