Figure 5.

Fine specificity of antibody responses. Sera from mice that were immunized IM thrice at a three-week interval with 10µg mRNA pfceltos UPenn N-glycosylation site modified (GM) in LNP1 (UPenn GM LNP1), or without N-glycosylation modification (UPenn LNP1) that were one-methylpseudouridine (m1Ψ)-5′-triphosphate, and cellulose affinity purified or with TriLink pfceltos mRNA that were N-glycosylation site modified (GM) in LNP1 (TriLink GM LNP1) and were ψ-pseudouridine and 5-methylcytosine modified were characterized for antibody specificities by ELISA against (A) PfCelTOS N-term, (B) CelTOS Peptide 1-2, (C) CelTOS Peptide 2-3, (D) PfCelTOS C-term, (E) CelTOS Peptide 4 and (F) Full length PfCelTOS protein (n = 15 per group). Antibody titers are reported as the geometric mean and 95% confidence interval of the dilution required to achieve an OD₄₀₅ = 1. Statistical analysis was performed using a Mann-Whitney test (*p<0.05, **p<0.01, ***p<0.001).