Table 1.

pfceltos mRNAs translation products.

Sequence Name	Signal Sequence	Coding Sequence	N-Glycosylation Sites
Mouse CH a PfCelTOS Wt-SS b	AUGAACGCGCUGCGC CGCCUGCCCGUGAUCUGCUCC UUCCUGGUGUUCCUGGUGUUCA GCAACGUGCUGUGC	MNALRRLPVICSFLVFLVFSNVLC C FRGNNGHNSSSSLYNGSQFIEQLNNSFTSAFLESQSMNKIGDDLAETISNELVSVLQKNSPTFLESSFDIKSEVKK HAKSMLKELIKVGLPSFENLVAENVKPPKVDPATYGIIVPVLTSLFNKVET AVGAKVSDEIWNYNSPDVSESEEESLSDDFFD	Native – 3 putative (NxS/T motif) d Residue Number 32:NSS e Residue Number 39:NGS f Residue Number 48: NNS g
Mouse CH PfCelTOS mouse-Ig Light Chain-SS	AUGAUGAGCCCAGC ACAAUUUCUCUUUCUUCUCG UACUCUGGAUACGAGAAACGAAUGGG	MMSPAQFLFLLVLWIRETNG FRGNNGHQSSSSLYQGSQFIEQ LQNSFTSAFLESQSMNKIGDDLAETISNELVSVLQKNSPTFLESSF DIKSEVKKHAKSMLKELIKVGLPSFENLVAENVKPPKVDPATYGIIV PVLTSLFNKVETAVGAKVSDEIWNYNSPDVSESEESLSDDFFD	Replace N>Q h at first position of the motif
Mouse CH PfCelTOS Human –IgE-SS	AUGGAUUGGACUUGGAUCCUGU UUCUGGUAGCAGCGGCUACUCG CGUACACUCC	MDWTWILFLVAAATRVHS FRGNNGHQSSSSLYQGSQFIEQLQNSFTSAFLESQSMNKIGDDLAETISNELVSVLQKNSPTFLESSFDI KSEVKKHAKSMLKELIKVGLPSFENLVAENVKPPKVDPATYGIIVP VLTSLFNKVETAVGAKVSDEIWNYNSPDVSESEESLSDDFFD	Replace N>Q at first position of the motif

^(a) Coding sequences were codon harmonized (CH) for optimal expression in mice.

^(b) Signal Sequence (SS).

^(c) Signal sequence amino acid shown as underlined text.

^(d) N-glycosylation sites shown as bold text.

^(e) NxS/T motif where N is Asparagine, X is any amino acid except Proline, S is Serine, and T is Threonine.

^(f) NSS motif where N is Asparagine and S is Threonine.

^(g) NGS motif where N is Asparagine, G is Glycine, and S is Threonine.

^(h) NNS motif where N is Asparagine and S is Threonine.

^(h) N>Q where N (Asparagine) is modified to Q (Glutamine).