Figure 1.

Single-molecule partition vs DNA input. Partitions with a single DNA molecule over all DNA-positive partitions are calculated based on Poisson statistics for a partition capacity of 20 K partitions common across commercially available dPCR platforms¹¹ versus 30 million partitions in UltraPCR’s. Low partition systems fall out of the single-molecule domain rapidly and require Poisson correction to derive quantitative accuracy up to a threshold number of counts.^20,21 In contrast, even at 1 million DNA input copies, UltraPCR still maintains the limited dilution regime, allowing for true single-molecule counting across a 6-log dynamic range.