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Orthogonal testing between UltraPCR and ddPCR. Scatterplots of RPP30 counts where the same serially diluted sets of samples were tested on (A) ddPCR with the Poisson 95% confidence interval (CI) as error bars as indicated by the manufacturer and (B) UltraPCR with positive partitions normalized to 50 μL. Note that “NTC” is defined as 0 expected RPP30 input from serial dilution but contains N1 input.
