Table 2.
Classes of Polymerase Errors within the HSV-tk target region
| Pol δ4 | Pol κ | Pol η | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
|
|
||||||||||
| FER1L4 | VEGF | VEGFmut | TAGLN2 | FER1L4 | VEGF | VEGFmut | TAGLN2 | FER1L4 | VEGF | VEGFmut | TAGLN2 | |
| TK a | 36 (0.95) | 25 (0.54) | 20 (0.30) | n.d. | 39 (0.76) | 133 (0.83) | 103 (0.82) | 26 (0.50) | 64 (0.67) | 118 (0.78) | 95 (0.82) | 37 (0.62) |
| Intra-G4 b | 2 (0.05) | 16 (0.35) | 18 (0.27) | n.d. | 11 (0.22) | 24 (0.15) | 19 (0.15) | 10 (0.19) | 28 (0.29) | 23 (0.15) | 10 (0.09) | 12 (0.20) |
| TK+G4 c | 0 | 5 (0.11) | 29 (0.43) | n.d. | 1 (0.02) | 4 (0.02) | 3 (0.02) | 16 (0.31) | 3 (0.03) | 10 (0.07) | 11 (0.09) | 11 (0.18) |
|
|
|
|
||||||||||
| Total | 38 | 46 | 67 | n.d. | 51 | 161 | 125 | 52 | 95 | 151 | 116 | 60 |
Counts of detectable polymerase errors (i.e., errors that resulted in a non-functional thymidine kinase), with proportions in parentheses, that are
a
confined to the HSV-tk sequence
b
confined to the G4 motif, or
c
large-scale deletions and errors that could not be unambiguously assigned to TK or intra-G4.
n.d., not determined