Figure 2.

Changes in bone morphology, reduced bone mechanical properties, and inhibition of bone formation in DIO. (A) The bone tissue of distal femoral metaphysis was observed by H&E staining. (B) Trabecular bone area ratio. (C) Osteoblast numbers. (D–G) Parameters of the three-point bending test, including the peak load, fracture load, maximum displacement, and stiffness. (H, I) Serum levels of bone turnover biomarkers, including CTX-I and PINP. Data are expressed as the mean ± SEM. n = 6, *P < 0.05, **P < 0.01, ns, no significance.