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. Author manuscript; available in PMC: 2022 Dec 29.
Published in final edited form as: Nat Methods. 2022 Feb 3;19(2):187–194. doi: 10.1038/s41592-021-01369-z

Extended Data Fig. 6 |. Web application allows easy piRNAi transgene design.

Extended Data Fig. 6 |

a. Screenshot from www.wormbuilder.org/piRNAi. Simple mode to target a single gene with pre-defined criteria. b. Advanced mode. In the advanced mode, several different piRNA clusters can be re-coded with either custom 20-mers (for example, targeting gfp) or by selecting all 20-mers at a given edit distance mapping to a selected gene isoform. c. Output file with the piRNAi transgene annotated in GenBank format. The sequence is displayed in ‘A plasmid Editor’ (ApE).