Skip to main content
. 2001 Feb;69(2):1044–1052. doi: 10.1128/IAI.69.2.1044-1052.2001

FIG. 3.

FIG. 3

Comparison of TLA-induced splenocyte responses of male WT and IL-5−/− mice infected with 10 T. gondii cysts by the oral route. Splenocytes were isolated from mice at 8 days postinfection and stimulated with 20 μg of TLA (closed bars) per ml or 1 μg of TLA (open bars) per ml for 96 h. Proliferation was assessed over the final 18 h by incorporation of tritiated thymidine (a). Supernatants were collected from parallel cultures after 72 h and analyzed for nitrite (b), IL-12 (c), IFN-γ (d), IL-4 (e), and IL-10 (f). No statistically significant differences were found (by Mann-Whitney U test) between the responses of WT and IL-5−/− mice. Results represent three replicate experiments.