Fig. 5.

Investigation of gene expression related with osteoblasts differentiation. The cells were incubated in the medium containing PBS, ac-LK (800 nM), Complex A (miRNA 2861, 50 nM and ac-LK, 300 nM), Complex B (miRNA 2861, 50 nM and ac-LK, 600 nM) and Complex C (Complex B and fRunx2 100 nM, LK 500 nM) for 24 h. Then the expression level of HDAC5 (A), Runx2 (B), OC (C) and ALP (D) were examined on 7, 14, and 21 days through qRT-PCR. The data represents the relative value compared with PBS, control group. This experiment was performed once