Figure 3.

DBD-Ext binds RNA hairpins. (A) Normalized FA binding of DBD-Ext to RNA from the DNA consensus sequence, rERE (red). rERE was made into a standard duplex (orange), the hairpin structure was rescued by the addition of a loop on the opposite end (green). (B) FA-binding curves of DBD-Ext bound to a hairpin from XBP1 (red), no internal bulge (green), and enlarged stem loops (purple and orange). DBD (C) or DBD-Ext (D) bound to labeled DNA-ERE and competed off by titration of unlabeled ERE DNA (red) or XBP1 RNA (blue). N ≥ 3, standard error of the mean reported.