Table 2. DoE Data.

std	run	flow rate	pH	column temperature	R1a	R2b	R3c
18	1	0.8	2.5	40	6.2	2.2	4.1
10	2	0.7	2.3	45	5.8	1.9	4.5
9	3	0.7	2.3	45	5.5	1.8	4.6
2	4	0.7	2.3	35	5.1	1.7	4.0
1	5	0.7	2.3	35	5.0	1.8	4.1
13	6	0.7	2.7	45	6.5	2.5	3.5
6	7	0.7	2.7	35	6.0	2.5	3.2
3	8	0.9	2.3	35	4.8	1.5	4.2
4	9	0.9	2.3	35	4.6	1.5	4.1
14	10	0.7	2.7	45	6.1	2.6	3.7
12	11	0.9	2.3	45	6.2	1.6	4.0
8	12	0.9	2.7	35	5.7	2.1	3.2
17	13	0.8	2.5	40	6.3	2.0	4.2
19	14	0.8	2.5	40	6.0	2.3	4.0
7	15	0.9	2.7	35	6.3	2.3	3.5
15	16	0.9	2.7	45	6.0	2.2	3.4
5	17	0.7	2.7	35	6.5	2.7	3.6
16	18	0.9	2.7	45	5.8	2.5	3.3
11	19	0.9	2.3	45	5.2	1.8	4.0

^aResolution between pibrentasvir and imp-D.

^bResolution between impurity A and nearby peak.

^cResolution between pibrentasvir and impurity at RRT ≈ 0.93.