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. 2022 Dec 14;11(24):e027228. doi: 10.1161/JAHA.122.027228

Figure 5. Validating the existence of discrete neutrophil subsets in murine hearts and human peripheral blood with flow cytometry.

Figure 5

A, Wild‐type mice were subjected to MI surgery for 1 and 7 days, or sham surgery for 7 days. The neutrophil subsets were identified by the expression of ICAM1 and Sell/Cd62L using flow cytometry. B, Quantification of A (n=4, 4, 4, 5, 5, 5, 5, 5, 5, respectively; 2‐way ANOVA followed by Bonferroni test). C, Neutrophils of PB were collected from sham, MI‐1D, and MI‐7D mice, and the frequency of ICAM+ or CD62L+ neutrophils among CD45+ cells were calculated. D, Quantification results of panel C (n=3, 3, 4, 4, 3, 3, respectively; 2‐way ANOVA followed by Bonferroni test). E, PB neutrophils from patients of control subjects and ACS were collected for flow cytometry analysis. F, The proportions of Neu_Icam1 and Neu_Sell in patient PB (n=4 for each group, 2‐way ANOVA followed by least significant difference test). ACS indicates acute coronary syndrome; MI, myocardial infarction; PB, peripheral blood; and PBMC, peripheral blood mononuclear cell.