TABLE 2.
Immune protection of guinea pigs immunized i.g. or i.n. against the intraconjunctival challenge with the wild-type S. flexneri 5 strain M90T
| Immunization strain | No. of animals inoculated | No. of animals showing the following ratingsa at hours p.i.
|
Protectionb %
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 48 h
|
72 h
|
96 h
|
|||||||||||||
| 0 | 1 | 2 | 3 | 0 | 1 | 2 | 3 | 0 | 1 | 2 | 3 | Full | Partial | ||
| i.g. routec | |||||||||||||||
| M90T | 10 | 1 | 4 | 5 | 0 | 1 | 4 | 5 | 0 | 1 | 4 | 5 | 0 | 10 | 40 |
| M90T aroC::Tn10 | 10 | 0 | 3 | 7 | 0 | 0 | 2 | 8 | 0 | 0 | 1 | 9 | 0 | 0 | 20 |
| M90T ΔpurE aroC::Tn10 | 10 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 100 | 0 |
| E. coli 395.1 | 10 | 0 | 0 | 10 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 9 | 1 | 0 | 0 |
| i.n. routed | |||||||||||||||
| M90T | 10 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 100 | 0 |
| M90T aroC::Tn10 | 10 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 100 | 0 |
| M90T ΔpurE aroC::Tn10 | 10 | 10 | 0 | 0 | 0 | 4 | 2 | 2 | 2 | 4 | 3 | 3 | 0 | 40 | 20 |
| E. coli 395.1 | 10 | 0 | 0 | 8 | 2 | 0 | 0 | 1 | 9 | 0 | 0 | 1 | 9 | 0 | 0 |
Reaction ratings: 0, normal; 1, palpebral edema with conjunctival hyperemia; 2, conjunctival hyperemia, plus slight exudate; 3, full-blown purulent keratoconjunctivitis. Vaccine significance efficacy was evaluated by Fisher's exact test.
Protection was evaluated at 72 h postchallenge and was defined as follows: full, percentage of eyes with rating of 0; partial, percentage of eyes with rating of 1.
i.g. route vaccine efficacy significance: M90T, full protection: P = 0.0909; partial protection: P = 0.0010; aroC, partial protection: P = 0.0152; M90T ΔpurE aroC::Tn10, full protection: P < 0.0001.
i.n. route vaccine efficacy significance: M90T, full protection: P = 0.0003; aroC, full protection: P = 0.0003; M90T ΔpurE aroC::Tn10, full protection: P = 0.0606.