FIG. 5.

PfR2Ha and -Hb do not colocalize with either PfAMA1, EBA175, or PfRAP1 by IFA analysis. Smears of free merozoites following schizont rupture of the D10 parasite are shown. Parasites were reacted with mouse antibodies to PfAMA1 (A), PfRAP1 (B), EBA175 (C), or PfRAP1 together with a rabbit antibody to PfR2Ha/Hb (2A11) (D). For images in the first column, the second antibody used was a sheep FITC-labeled anti-rabbit antibody; for images in the second column, the second antibody used was a goat rhodamine-labeled anti-mouse antibody; the Merge column shows the red and green images overlaid. Magnifications: A to C, ×1,000; D, ×3,000. The outline of three merozoites is shown in panel D.