Figure 2.

Hepatic fibrosis is ameliorated in multidrug-resistant 2 knockout (Mdr2^−/−) mice after apical sodium bile acid (BA) transporter (ASBT) Vivo-Morpholino (VM). We found increased collagen deposition shown by Fast Green-Sirius Red (FG-SR) staining and semiquantification (A) and confirmed with Masson’s trichrome (B) in Mdr2^−/− Control mice compared with wild-type (WT) mice. ASBT VM treatment reduced collagen deposition in Mdr2^−/− mice (A and B). Hepatic Fn1 expression increased in Mdr2^−/− Control mice but was unchanged in WT mice and was reduced after ASBT VM (C). Representative images are presented as ×10. Data are presented as mean ± SD of 5 images for FG-SR semiquantification and 4 experiments for qPCR from 7 to 10 mice per group. **P < 0.01, ***P < 0.001, ****P < 0.0001.