Figure 7.
Klotho more effectively ameliorated high dietary phosphate-induced than vascular endothelial growth factor receptor type 2 (VEGFR2) haploinsufficiency-associated peritubular capillary rarefaction. A: mice with four different genotypes [wild-type (WT), VEGFR2+/−, Tg-kl, and Tg-kl;VEGFR2+/−] at 3-mo old were fed normal- or high-phosphate diet for 12 wk, respectively. Each treatment per genotype had 10 mice. B: plasma creatinine (Cr). C: plasma Pi. D: immunoblot analysis for endothelial marker (CD31), Klotho, VEGFR2, and β-actin in total kidney lysates. D, top: representative blots. D, bottom: quantitative analysis of all immunoblots from the four groups. B–D: quantitative data are expressed with scatterplots of individual data points (open circles indicate male mice and pink circles indicate female mice) and means ± SD (bars and errors) of mice from each group. Statistical significance was evaluated by two-way ANOVA followed by a Student–Newman–Keuls test. Significant differences were accepted when *P < 0.05 and **P < 0.01 between two groups within the same phosphate diet treatment and $P < 0.05 and $$P < 0.01 between normal- and high-phosphate diet within the same genotype. The sample number in each group is presented in parentheses underneath each corresponding bar.