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. Author manuscript; available in PMC: 2023 Jun 19.
Published in final edited form as: Nat Metab. 2022 Dec 19;4(12):1792–1811. doi: 10.1038/s42255-022-00706-6

Extended Data Fig. 8.

Extended Data Fig. 8

(A) Immunoblot for TFEB, TFE3, and proteins corresponding to their transcriptional target genes in Tsc2−/− MEFs treated for two days with control siRNA, siTfeb, or siTfe3.

(B,C) 14C-TGs assayed by pulse-chase with [1-14C]-oleate tracer (6 h) in Tsc2−/− MEFs treated as in (A) followed by chase in cold medium for 16 hrs with vehicle or 250 nM torin1, graphed as mean ± SD relative to control siRNA-treated cells (B) and relative to vehicle-treated cells (C), n=3.

Statistical analysis by one-way ANOVA (B) and two-way ANOVA (C). n.s., not significant.