TABLE 2.
Effect of glucose availability on the production of toxins and spreading factors in continuous cultures of C. septicum
| Db (h−1) | Tdc (h) | Culture conditions | Cell count (ml−1) | Activity (U/mg [dry wt] of cells) ofa:
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| Hemolysin | DNase | Hyaluronidase
|
Neuraminidase
|
||||||
| Cell-bound | Extracellular | Cell-bound | Extracellular | ||||||
| 0.02 | 34.7 | Glucose excess | (4.0 ± 1.2) × 108 | NDd | 4.5 ± 2.7 | 14.2 ± 3.8 | 55.8 ± 3.4 | 360 ± 20.3 | 270 ± 24.3 |
| Glucose limited | (2.3 ± 1.2) × 108 | ND | 25.2 ± 3.6 | ND | 14.5 ± 2.1 | 89.6 ± 6.6 | 55.8 ± 4.8 | ||
| 0.10 | 6.9 | Glucose excess | (3.6 ± 0.5) × 108 | ND | 1.1 ± 0.4 | 2.1 ± 1.1 | 22.7 ± 2.9 | 200 ± 18.2 | 150 ± 12.1 |
| Glucose limited | (5.9 ± 0.8) × 108 | ND | 8.3 ± 1.9 | ND | ND | ND | ND | ||
| 0.36 | 1.9 | Glucose excess | (9.2 ± 1.1) × 107 | 34.5 ± 5.6 | ND | ND | ND | ND | ND |
| Glucose limited | (6.7 ± 2.1) × 108 | 15.2 ± 3.4 | ND | ND | ND | ND | ND | ||
| 0.65 | 1.1 | Glucose excess | (8.8 ± 1.4) × 107 | 108 ± 12.7 | ND | ND | ND | ND | ND |
| Glucose limited | (6.8 ± 0.7) × 108 | 82.6 ± 8.5 | ND | ND | ND | ND | ND | ||
a
Results are means ± standard deviations of data obtained in three separate experiments.
b
Dilution rate.
c
Cell doubling time, calculated as 0.693/D.
d
ND, not detected.