TABLE 3.

Formation of toxins and mucinolytic enzymes in continuous cultures of C. septicum grown in mucin-limited chemostats^a

Db (h−1)	Cell count (ml−1)	Cytotoxicity titer	Activity (U/mg [dry wt] of cells) of:
			Hemolysin	Glycosulfatase	Neuraminidase		N-Acetyl β-glucosaminidase		β-Galactosidase		Hyaluronidase
					Cell bound	Extracellular	Cell bound	Extracellular	Cell bound	Extracellular	Cell bound	Extracellular
0.04	(6.6 ± 0.8) × 108	16	NDc	5.2 ± 0.1	1118 ± 56	73 ± 8.1	3.3 ± 0.13	0.6 ± 0.20	2.6 ± 0.20	0.2 ± 0.02	58.3 ± 6.0	198 ± 12.3
0.16	(6.7 ± 0.2) × 108	32	2.5 ± 0.4	5.0 ± 0.3	921 ± 43	13 ± 1.9	4.0 ± 0.10	2.1 ± 0.11	3.7 ± 0.20	0.3 ± 0.03	163 ± 13.2	143 ± 11.7
0.23	(6.8 ± 0.3) × 108	16	16.6 ± 0.2	0.4 ± 0.1	1462 ± 71	ND	5.0 ± 0.24	0.5 ± 0.02	1.5 ± 0.06	0.2 ± 0.02	99.5 ± 7.0	127 ± 15.1
0.36	(7.1 ± 0.1) × 108	16	109 ± 6.0	ND	956 ± 35	ND	4.4 ± 0.16	0.5 ± 0.03	0.8 ± 0.02	0.6 ± 0.04	ND	121 ± 11.0
0.63	(8.2 ± 0.4) × 108	16	256 ± 10.6	ND	1186 ± 92	ND	5.0 ± 0.24	0.9 ± 0.05	0.9 ± 0.06	0.5 ± 0.01	ND	ND

^aResults are means ± standard deviations of data obtained in three separate experiments. 

^bDilution rate. 

^cND, not detected.