FIG. 2.

Flow cytometric analysis of lung dendritic cells after overnight culture of nonadherent CD11c⁺ lung cells. Cells were washed and stained with CD11c⁺ -FITC and I-A^b–biotin plus avidin PerCP. The left panel shows the variable size of the CD11c⁺ cells. Cells could be gated into three populations as follows: region 1 (R1) with FSC^low and SSC^low corresponding to small cells; region 2 (R2) with FSC^mid and SSC^low corresponding to large cells with low granularity; and region 3 (R3) with FSC^high and SSC^high corresponding to larger and more granular cells. The panels on the right side represent the expression of CD11c⁺-FITC (x axis) and I-A^b–PerCP (y axis). The expression of these antigens on each of the different populations is shown in region 1 (upper dot plot was gated in R1), region 2 (bottom dot plot was gated in R2), and region 3 (middle dot plot was gated in R3). According to their cell surface expression of CD11c and I-A^b antigens, purified lung dendritic cells after overnight culture constitute a mixed population of cells and can be classified in three populations: (R1 population) I-A^{b low} CD11c^{+ low} expression, FSC^low and SSC^low; (R2 population) I-A^{b high} and CD11^{+ low}, FSC^mid SSC^low; and (R3 population) I-A^{b low} CD11c^{+ high}, FSC^high and SSC^high.