FIG. 3.

Cytokine production by CD4⁺ Th cells from BL/6 and CBA mice stimulated with the 26-kDa antigen. CD4⁺ Th cells were isolated from mesenteric lymph nodes of 8.5-week-infected mice and cultured with the indicated antigens in the presence of APC as described in Materials and Methods. The cytokines were measured in culture supernatants by ELISA. Data are expressed as means of triplicate determination ± 1 standard deviation. Also shown for comparison are responses to Sm-p40 and SEA. The experiments were repeated at least three times with similar results.