FIG. 1.
Changes in light scatter characteristics (A, B, and C) and cell surface expression of MHC class II molecules (D) on PBMC exposed to Al(OH)3 and to GM-CSF plus IL-4. Isolated PBMC were cultured for 24 (A), 48 (B), and 72 (C) h with medium alone, Al(OH)3, or GM-CSF plus IL-4; they were then harvested, washed, and analyzed by flow cytometry. Light scatter plots show the placement of gates for subsequent analysis of fluorescent antibody staining. FSC, forward scatter; SSC, side scatter. (D) Mean fluorescence intensity of cells stained with FITC-conjugated MAbs to MHC class II within a monocyte gate (big granular cells) exposed to medium alone (○) or Al(OH)3 at 1 μg/ml (▪), 5 μg/ml (●), or 50 μg/ml (×) during culturing (results of one representative experiment).