Table 1.
Antibodies used in flow cytometry.
| Surface markers | |||
| mAB | Clone | Manufacture | Isotype |
| HLA-DR-APC | Tü36 | BioLegend, San Diego, USA | Mouse IgG2b, k |
| CD11b-FITC | 94 | Beckman coulter | Mouse, IgM |
| CD14-PerCP-Cy5.5 | Mφp9 | BD Biosciences, USA | Mouse, IgG2b,k |
| CD15-PE | H198 | eBioscience, San Diego, USA | Mouse, IgM,k |
| CD4-PE | RPA-T4 | Immunostep, Salamanca, Spain | Mouse IgG1 |
| CD8-APC | SK1 | BioLegend, San Diego, USA | Mouse,IgG1,k |
| CD25-PE-Cy5.5 | PC61.5 | eBioscience, San Diego, USA | Rat/IgG1, lambda |
| Intracellular markers | |||
| mAB | Clone | Manufacture | Isotype |
| Foxp3-APC | 236A/E7 | eBioscience, San Diego, USA | Mouse, IgG1,k |
| IFN-γ-PerCP | XMG1.2 | BioLegend, San Diego, USA | Rat, IgG1, k |
| TGF-β-PE | TW4-9E7 (RUO) | BD Biosciences, USA | Mouse, IgG1,k |