Component	Amount (μL)	Final concentration
Annealed spacer oligonucleotides, 1μM Golden Gate part 1 (from Step 3Aiv)	1 μl	0.1 μM
Annealed and phosphorylated sgRNA scaffold oligonucleotides, 1μM Golden Gate part 2 (from Step 3Aiv or 3Avi)	1 μl	0.1 μM
Annealed epegRNA RTT/PBS 3′ extension oligonucleotides, 1μM Golden Gate part 3 (from Step 3Aiv)	1 μl	0.1 μM
pU6-tevopreq1-GG-acceptor 2.2 kb fragment, 30ng / μl Golden Gate part 4 (from Step 3Axii)	1 μl	3 ng / μl
BsaI-HFv2 (20U / μl)	0.2 μl	0.4 U/μl
NcoI-HF (20U / μl)	0.2 μl	0.4 U/μl
PvuII-HF (20U / μl)	0.2 μl	0.4 U/μl
T4 DNA ligase (40U / μl)	0.50 μl	2 U/μl
10× T4 DNA ligase buffer	1 μl	1×
Nuclease-free H20	3.9 μl	-
Total reaction volume	10 μl	-