| Component | Amount (μL) | Final concentration |
|---|---|---|
| Isothermal assm. Forward primer, 100 μM | 0.25 μl | 0.5 μM |
| Isothermal assm. Reverse primer, 100 μM | 0.25 μl | 0.5 μM |
| pU6-tevopreq1-GG-acceptor, 200 ng/μl | 0.05 μl | 0.2 ng / μl |
| Phusion HF 2× Master Mix | 25 μl | 1× |
| Nuclease-free H20 | 24.5 μl | - |
| Total reaction volume | 50 μl | - |
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