| Component | Amount (μL) | Final concentration |
|---|---|---|
| NEBuilder HiFi DNA Assembly 2× Master Mix | 6 μl | 1× |
| Gene fragment (from Step 3Bi), 10 ng/μl | 5 μl | 4.2 ng/μl |
| 2 kb PCR amplicon (from Step 3B(vi), 70 ng/μl | 1 μl | 5.8 ng/μl |
| Total reaction volume | 12 μl | - |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.