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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Nat Protoc. 2022 Aug 8;17(11):2431–2468. doi: 10.1038/s41596-022-00724-4
Component Amount (μL) Final concentration
in vitro transcription forward primer, 100 μM 0.75 μl 0.25 μM
in vitro transcription reverse primer, 100 μM 0.75 μl 0.25 μM
mRNA transcription template plasmid, 40 ng/μl 6 μl 0.8 ng/μl
Phusion U Green Multiplex Master Mix, 2× 150 μl 1×
Nuclease-free H20 142.5 μl -
Total reaction volume 300 μl -