| Cycle number | Denature | Anneal | Extend |
|---|---|---|---|
| 1 | 98 °C, 2 m | - | - |
| 2–36 | 98 °C, 15 s | 71.4 °C, 30 s | 72 °C, 3:30 m (PEmax); 72 °C, 1:15 m (MLH1dn) |
| 37 | - | - | 72 °C, 5 m |
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