Component	Amount (μL)	Final concentration
Nuclease-free H20	24.4 - x μl	-
10X Reaction Buffer	2 μl	0.5×
ATP, 100 mM	2 μl	5 mM
CTP, 100 mM	2 μl	5 mM
GTP, 100 mM	2 μl	5 mM
N1-Methylpseudouridine-5′-Triphosphate, 100 mM	2 μl	5 mM
CleanCap Reagent AG, 100mM	1.6 μl	4 mM
Purified linear Template DNA from Step 6	x μl	1 μg total
T7 RNA polymerase mix	4 μl	-
Total reaction volume	40 μl	-