| Component | Amount (μL) | Final concentration |
|---|---|---|
| Step 9 reaction mix | 40 μl | - |
| Nuclease-free H20 | 136 μl | - |
| DNAse I reaction buffer, 10× | 20 μl | 1× |
| DNAse I, RNAse free 2 U/μl | 4 μl | 0.04 U/μl |
| Total reaction volume | 200 μl | - |
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