| Component | Amount per well | Volume (μl) per well |
|---|---|---|
| Prime editor vector (Step 20A(ii)) | 200 ng | variable |
| epegRNA vector (Step 3a or 3b) | 66 ng | variable |
| Nicking sgRNA vector (Step 3a or 3b) | 22 ng | variable |
| MLH1dn vector (Step 20A(ii)) | 100 ng | variable |
| Opti-MEM Reduced Serum Medium (Thermo Fisher Scientific) | - | to 5 μl |
| Total reaction DNA and volume | 388 ng | 5 μl |
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