| Component | Amount | Volume (μl) |
|---|---|---|
| Prime editor in vitro transcribed mRNA (2 μg/μl stock) (Step 19) | 1 μg | 0.5 μl |
| epegRNA synthetic RNA (200μM stock) (Step 3C) | 90 pmol | 0.45 μl |
| Nicking sgRNA synthetic RNA (100μM stock) (Step 3C) | 60 pmol | 0.6 μl |
| MLH1dn in vitro transcribed mRNA (2 μg/μl stock) (Step 19) | 1 μg | 0.5 μl |
| Fibroblasts in Lonza SE buffer (5000 cells/μl, from Step 20Bxi) | 1 × 105 cells | 20 μl |
| Total reaction volume | - | 22.05 μl |
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