| Component | Amount (μL) | Final concentration |
|---|---|---|
| Phusion U Green Multiplex Master Mix, 2× | 12.5 μl | 1× |
| PCR1 forward primer (Step 27, Table 2), 100 μM | 0.125 μl | 0.5 μM |
| PCR1 reverse primer (Step 27, Table 2), 100 μM | 0.125 μl | 0.5 μM |
| Lysis mix with harvested gDNA (Step 26) | 1 μl | - |
| Nuclease-free H20 | 11.25 μl | - |
| Total reaction volume | 25 μl | - |
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