| Cycle number | Denature | Anneal | Extend |
|---|---|---|---|
| 1 | 98 °C, 3 m | - | - |
| 2–7 | 98 °C, 10 s | 60 °C, 20 s | 72 °C, 30 s |
| 8 | - | - | 72 °C, 5 m |
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