| Component | Amount (μL) | Final concentration |
|---|---|---|
| Top oligonucleotide, 100 μM | 1 μl | 4 μM |
| Bottom oligonucleotide, 100 μM | 1 μl | 4 μM |
| Annealing buffer | 23 μl | - |
| Total reaction volume | 25 μl | - |
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