| Component | Amount (μL) | Final concentration |
|---|---|---|
| 4 μM dsDNA Golden Gate part 2 (not phosphorylated) | 25 μl | 1 μM |
| 10× T4 DNA ligase buffer | 10 μl | 1× |
| T4 Polynucleotide Kinase (10U / μl) | 2 μl | 0.2 U / μl |
| Nuclease-free H20 | 63 μl | - |
| Total reaction volume | 100 μl | - |
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