| Component | Amount (μL) | Final concentration |
|---|---|---|
| pU6-tevopreq1-GG-acceptor, 5μg | x μl | 125 ng/μl |
| BsaI-HFv2 (20U / μl) | 1 μl | 0.5 U/μl |
| NcoI-HF (20U / μl) | 1 μl | 0.5 U/μl |
| PvuII-HF (20U / μl) | 1 μl | 0.5 U/μl |
| 10× rCutsmart Buffer | 4 μl | 1× |
| Nuclease-free H20 | to 40 μl | - |
| Total volume | 40 μl | - |
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