Table 1.

Cucumber reference genes information and their primer sequences.

Gene	Accession number	Annotation	Gene ID in cucumber	Forward primer	Reverser primer	References
ACT	AB010922	Actin	Csa6G484600	CCGTTCTGTCCCTCTACGCTAGTG	GGAACTGCTCTTTGCACTCTCGAG	Niu et al., 2018; Wen et al., 2019; Zhang et al., 2021; Qin et al., 2021
TUA	AJ715498	Alpha-tubulin	Csa4G000580	CATTCTCTCTTGGAACACACTGA	TCAAACTGGCAGTTAAAGATGAAA	Lü et al., 2017; Sun et al., 2019
UBC		Ubiquitin conjugating enzyme	Csa3G358610	GTCACCATTCATTTTCCTCCG	GGGCTCCACTGCTCTTTCA	Song et al., 2019
EF1	EF446145	Elongation factor 1-alpha	Csa2G139820	ACTTTATCAAGAACATGATTAC	TTCCTTCACAATTTCATCG	Wang et al., 2015; Zhang et al., 2019
CYP	AY942800	Cyclophilin	Csa7G009740	TTTCATGTGCCAGGGAGG	AGCCAATCGGTCTTAGCG	Le et al., 2012; Olaetxea et al., 2015
PRL36Aa	HM594174	60S ribosomal protein L36a/L44	Csa3G653380	AAGATAGTCTTGCTGCACAGGG	AACACGGGCTTGGTTTGA	Miao et al., 2019
PP2A	HM594171	protein phosphatase 2A regulatory subunit A	Csa5G608520	GAAGCTGTAGGACCTGAACCA	AGCCGCTGCAATACGAAC	Petriccione et al., 2015
UBI	AF104391	Ubiquitin-like protein	Csa2G036600	CCTTATTGACCAACCAGTAGT	GGACAATGTTGATTTCCTCG	Wen et al., 2019; Zhao et al., 2019; Shen et al., 2019
CACS	GW881874	Clathrin adaptor comple subunit	Csa3G902930	TGGGAAGATTCTTATGAAGTGC	CTCGTCAAATTTACACATTGGT	Migocka et al., 2011; Rajsz et al., 2016
UBQ	AY372537	Polyubiquitin	Csa4G089780	CACCAAGCCCAAGAAGATC	TAAACCTAATCACCACCAGC	Wei et al., 2016; Kopczewski et al., 2022
F-BOX	GW881870	F-box protein	Csa5G642160	GGTTCATCTGGTGGTCTT	CTTTAAACGAACGGTCAGTCC	Rodrigues et al., 2010; Le et al., 2012
YSL8	GW881872	mitosis protein	Csa5G175720	CCTTGTGGATATCACAGAAGTT	CTTGTTTATCCTTGAGTGCC	Song et al., 2019
PDF2	GW881868	Protein phosphatase 2	Csa5G608520	GTAGGACCTGAACCAACTA	CTTCACGCAGGGAAGA	Chen et al., 2017