TABLE 2.
PCR primers used for this study
| Primer name | Gene or designation | Direction | Location in genec | GenBank accession no. | Sequence (5′-3′)b |
|---|---|---|---|---|---|
| C6778 | babA (HP1243) | Forward | 570–588 | AE000629 | GCTTACCCGCGCTCAAAG |
| C6779 | babA (HP1243) | Reverse | 1056–1076 | AE000629 | CTCCGTGAAAGGGTTGAAAG |
| B1999 | babA (HP1243) | Reverse | 1088–1107 | AE000629 | GTTAAGCGAGCATGCCTTG |
| BA909 | babA (HP1243) | Forward | 410–428 | AE000629 | CTTCAACCACCATCTTCA |
| B1998 | babA (95-76)a | Forward | 562–580 | NAa | GCTTGCCAGCGCTCAACC |
| AN5036 | babB (HP896) | Forward | 451–469 | AE000599 | ACCATCACTTGCAATTCG |
| AN5037 | babB (HP896) | Reverse | 1042–1060 | AE000599 | GAGCGTTTTTGAGCATGC |
| B9617 | babA (HP1243) | Forward | 1–23 | AE000629 | ATGAAAAAACACATCCTTTCAT |
| AN5954 | babA (JHP833) | Reverse | 2213–2235 | AE001512 | TTAGTAAGCGAACACGTAATTC |
| C5773 | babB (HP896) | Forward | 1–20 | AE000599 | ATGAAAAAAACCCTTTTAC |
| C5774 | babB (HP896) | Reverse | 2138–2156 | AE000599 | TTAGTAAGCGAACACATA |
| AN5045 | JHP834 | Forward | 1–19 | AE001512 | GTGTGCGGCGTATTATCG |
| AN5043 | HP1244 | Forward | 1–21 | AE000629 | ATGGAAAGAAAACGCTATTC |
| C7491 | pUC4K | Forward | 369–401 | X06404 | GCTCTAGAGCTCACGACGTTGTAAAACGACGGCCAGTG |
| C7492 | pUC4K | Reverse | 1598–1638 | X06404 | GCTCTAGAGCGTTGTGTCTCAAAATCTCTGATGTTAC |
| AN5485 | pUC4K | Forward | 369–401 | X06404 | CGCGGATCCGCGTCACGACGTTGTAAAACGACGGCCAGTG |
| AN5486 | pUC4K | Reverse | 1598–1638 | X06404 | CGCGGATCCGCGGTTGTGTCTCAAAATCTCTGATGTTAC |
a
NA, not available, unpublished data.
b
Underlined sequences correspond to XbaI and BamHI restriction sites.
c
Nucleotide positions.