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Peripheral blood lymphocytes (PBMCs) were prepared at 2, 4, 6 and 8 weeks post primary immunization and cultured with a truncated HA2 peptide and Con A (5 μg/mL). Lymphocyte proliferation was measured by cell counting kit-8 (CCK-8) reagent and shown as the stimulation index. (A) PBMCs stimulated by truncated HA2 peptide. (B) PBMCs stimulated by Con (A) Values represent mean ± S.D. (n = 3). *p < 0.05 was significantly different.
