Figure 2.

Verification of lcoET3 transgene DNA in gene-modified PLCs and hLSECs using PCR. DNA was extracted from control and gene-modified cells (3 passages after transduction/transfection) and subjected to PCR with primers designed to amplify a 395-bp region within the lcoET3 sequence. Agarose gel electrophoresis was then performed to visualize the size of the PCR products. Unmodified control cells and a reaction containing all constituents of the PCR except for template DNA (water/no-template) were used as negative controls while LV-lcoET3 cells and pure lcoET3 plasmid were used as positive controls.