Figure 5.
Evaluation of FVIII coagulation activity by activated partial thromboplastin time (aPTT) assay performed on 24h culture supernatants after normalization for cell number in each sample. Data are presented as the amount of functional FVIII in International Units (IU) being produced by 106 cells in 24h. (A – PLCs, B – hLSECs). n = 7 for unmodified/control PLCs, n = 3 for LV-lcoET3 PLCs, n = 12 for CRISPR-edited PLCs, and n = 5 for Plasmid-lcoET3-transfected PLCs; n = 7-9 for all hLSEC groups. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.