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. 2001 Feb;69(2):1181–1184. doi: 10.1128/IAI.69.2.1181-1184.2001

FIG. 2.

FIG. 2

Expression of ToxR-TM-MBP fusion proteins. E. coli strains MM39 (lane a), MM39 pccGpA-wt (lane b), MM39 pccGpA-G83I (lane c), MM39 pccVacA-wt (lane d), and MM39 pccVacA-G14A (lane e) were cultured in Luria-Bertani medium to an optical density at 600 nm of approximately 0.35. Equal culture volumes were pelleted, lysed in sodium dodecyl sulfate-sample buffer, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotted using anti-MBP antisera (New England Biolabs). The 65-kDa ToxR-TM-MBP fusion proteins are indicated by the arrow. Sizes are indicated in kilodaltons on the left.