Figure 2.

Differences in transfection efficiency and transcriptomes between cell lines transfected with lipofectamine LTX in SFMd

(A) Transfection efficiency (%GFP⁺ cells) and transgene expression levels (mean GFP) for each cell line at 24 h after transfection with lipofectamine LTX and pEF-GFP pDNA in SFM. Representative flow cytometry histograms and fluorescent microscopy images for each cell line are also shown in Figure S1. Error bars indicate standard deviation of the triplicate samples. (B) Total number of upregulated and downregulated DEGs observed in each cell line after lipofection in SFM. DEGs were defined as having at least a 2-fold change in TPM that was statistically significant (p_adj < 0.05) over the course of three independent experiments. (C–F) Gene expression levels (TPMs) in HEK-293T cells (C), PC-3 cells (D), Jurkat T cells (E), and primary CD3⁺ T cells (F) that were either transfected with lipofectamine LTX (y axis) or not transfected (x axis). All TPM values are averaged from three separate transfections and corresponding mRNA-sequencing experiments. Green triangles indicate genes that were significantly upregulated in transfected cells (p_adj < 0.05), red circles indicate downregulated genes (p_adj < 0.05), and gray squares represent unaffected genes.