Figure 3.

Effects of serum and electroporation on transfection efficiency and the PC-3 transcriptome

(A) Effects of serum on IFN λ1/3 expression in PC-3 cells. Asterisks (∗) indicate significant (p < 0.05 as determined by Student’s t-test) increases in IFNλ levels after transfection relative to untransfected control cells, while carets (ˆ) indicate significant increases between ELISA measurements at 6 and 24 h after transfection. (B) Transfection efficiencies (green bars, left axis) and the number of significantly upregulated genes (red bars, right axis) observed after transfecting PC-3 cells with lipofectamine in SFM or SCM or electroporation in SCM. Asterisks indicate significant differences in transfection efficiency (∗significantly higher than lipofection in SFM; ∗∗significantly higher than lipofection in SFM and SCM, p < 0.05 as determined by Student’s t-test). (C/D) Gene expression levels (TPM) for PC-3 cells that were lipofected (C) or electroporated (D) in SCM. All TPM values are averaged from three separate transfections and corresponding mRNA-sequencing experiments. Green triangles indicate genes that were significantly upregulated in transfected cells, red circles indicate downregulated genes, and gray squares represent unaffected genes. All error bars indicate the standard deviation of the measurements for each bar.