Figure 4.

Effects of pDNA amounts during transfection on the PC-3 transcriptome

(A) Transfection efficiencies obtained with PC-3 cells using lipofectamine (2.75 μL/well) and pDNA (0.1 or 1.0 μg), measured at either 1 or 2 days after transfection. Error bars indicate the standard deviation from each triplicate of samples. (B) A summary of the number of genes that were upregulated (green bars) or downregulated (red bars) after each type of transfection. ∗Significant differences in transfection efficiency. (C) Gene expression levels (TPM) for PC-3 cells that were lipofected with either 0.1 or 1.0 μg pDNA. Green triangles indicate genes that were significantly upregulated in the cells that received the higher dose of pDNA, while red circles indicate genes that were downregulated in those cells.